Course Code: BIOT 202
Academic Year: 2024-2025
This course introduces terminologies and concepts fundamental to the field of molecular biology. Students explore essential concepts at the molecular level such as the chemistry of the cell, cell structure and function, membrane properties, transport across cellular membranes, and genes as blueprints of life. Learners in this course are introduced to the theory and techniques associated with isolation, purification, manipulation, and characterization of nucleic acids. Topics include the isolation of RNA and DNA, restriction endonuclease digestion, plasmid mapping, hybridization analysis, construction of recombinant plasmids, polymerase chain reaction, and transformation of bacteria using plasmid DNA.